Prevalence of CTX-M Gene as an ESBL Marker in Clinical Klebsiella pneumoniae Isolates

Arafah Nurfadillah; Wahdaniar; Ummu Syauqah Al Musyahadah; Nur Insani Amir; Ade Irma; Miladiarsi; Tenri Ayu Adri

doi:10.56741/hesmed.v2i01.164

Authors

Arafah Nurfadillah
arafahnurfadillah@gmail.com
Universitas Megarezky, Indonesia
Wahdaniar Universitas Megarezky, Indonesia
Ummu Syauqah Al Musyahadah Universitas Megarezky, Indonesia
Nur Insani Amir Universitas Megarezky, Indonesia
Ade Irma Universitas Megarezky, Indonesia
Miladiarsi Universitas Megarezky, Indonesia
Tenri Ayu Adri Universitas Megarezky, Indonesia

DOI:

https://doi.org/10.56741/hesmed.v2i01.164

Keywords:

Antibiotic resistant, CTX-M gene, Klebsiella pneumoniae

Abstract

Klebsiella pneumonia is a bacteria that causes many negative impacts on health, primarily due to its tendency to develop antibiotic resistance. This research aims to identify the prevalence of the CTX-M gene as an ESBL marker of the Clinical Isolate of K. pneumoniae of Wahidin Sudirohusodo General Hospital. The samples consisted of 50 Clinic isolates of K. pneumoniae which fulfilled the inclusion and exclusion criteria. The research procedure consisted of DNA extraction, amplification, and agarose gel electrophoresis. The research results indicate that 38 samples (76%) are positive for the CTX-M gene, while 12 samples (24%) are negative or not found CTX-M gene. Thus, the prevalence of the CTX-M gene in K. pneumoniae resistant to beta-lactam antibiotics in Wahidin Sudirohusodo General Hospital is relatively high.

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Author Biographies

Arafah Nurfadillah, Universitas Megarezky, Indonesia

Arafah Nurfadillah is a lecturer at Bioinformatics Departement, Faculty of Health Technology, Universitas Megarezky, Indonesia. Her specialization is microbiology and biochemistry. She has published many articles in antibiotic resistance of pathogenic bacteria (email: arafahnurfadillah@unimerz.ac.id).

Wahdaniar, Universitas Megarezky, Indonesia

Wahdaniar is a lecturer at Biomedical Science Department. She is a secretary of Biomedical Science Study Program at Megarezky University. She is a journal reviewer for Journal of Health Sciences and Medical Development (email: niarwahdaniar03@unimerz.ac.id).

Ummu Syauqah Al Musyahadah, Universitas Megarezky, Indonesia

Ummu Syauqah Al Musyahadah is a lecturer at Bioinformatics Departement, Faculty of Health Technology, Universitas Megarezky, Indonesia. She has interest in making science applicable to the community (email: syauqah.almusyahadah@unimerz.ac.id).

Nur Insani Amir, Universitas Megarezky, Indonesia

Nur Insani Amir is a lecturer at Bioinformatics Departement, Faculty of Health Technology, Universitas Megarezky, Indonesia. Her research interest is in natural product for anticancer compounds. (email: nurinsaniamir@unimerz.ac.id).

Ade Irma, Universitas Megarezky, Indonesia

Ade Irma is a lecturer at Biomedical Science Department, Megarezky University. Her position is as Quality Control Taskforce in this university. She is active to promote education quality in health and medical sciences. (email: adeirmabio93@gmail.com).

Miladiarsi, Universitas Megarezky, Indonesia

Miladiarsi is a lecturer at Biomedical Science Department. She is Head of Biomedical Science Study Program at Megarezky University. Her research interest is in medical microbiology, and genetic epidemiology (email: miladiarsi.bio09@gmail.com).

Tenri Ayu Adri, Universitas Megarezky, Indonesia

Tenri Ayu Adri is a lecturer at Pharmacy Department, Megarezky University. Her position is as Head of Quality Assurance Unit at this department. She is active in many profesional association. (email: farmakologitenriayu@unimerz.ac.id).

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