Tem Gene Detection as ESBL Marker in Clinical Isolates Klebsiella pneumoniae : A Case Study

Arafah Nurfadillah; Wahdaniar; Miladiarsi; Ade Irma; Tenri Ayu Adri

doi:10.56741/hesmed.v1i02.155

Authors

Arafah Nurfadillah Universitas Megarezky
Wahdaniar
niarwahdaniar03@gmail.com
Universitas Megarezky
Miladiarsi Universitas Megarezky
Ade Irma Universitas Megaresky
Tenri Ayu Adri Universitas Megarezky

DOI:

https://doi.org/10.56741/hesmed.v1i02.155

Keywords:

ESBL, Klebsiella pneumoniae, PCR, Tem Gene

Abstract

Klebsiella pneumoniae is one of the most common ESBL-producing microorganisms. The research aims to detect the Tem gene in clinical isolates of K. pneumonaie from the Laboratory of clinical pathology of Wahidin Sudirohusodo General Hospital. Methods: This research is a descriptive cross-sectional design. Fifty samples of K. pneumonieae were obtained from the Laboratory of clinical pathology from March to May 2018. Tem gene was detected using primary Tem 164.SE and Tem 165.AS (445 bp) of Polymerase Chain Reaction (PCR). The research results indicate that 48 isolates are positive, containing the Tem gene with the highest number found in the sputum specimen. Genotype detection of bacteria-producing ESBL is essential so that antibiotic therapy given to the patients is more effective and efficient.

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Author Biographies

Arafah Nurfadillah, Universitas Megarezky

Arafah Nurfadillah is a lecturer at Bioinformatics Departement, Faculty of Health Technology, Universitas Megarezky, Indonesia. Her specialization is microbiology and biochemistry. She has published many articles in antibiotic resistance of pathogenic bacteria (email: arafahnurfadillah@unimerz.ac.id).

Wahdaniar, Universitas Megarezky

Wahdaniar is a lecturer at Biomedical Science Department. She is a secretary of Biomedical Science Study Program at Megarezky University. She is a journal reviewer for Journal of Health Sciences and Medical Development (email: niarwahdaniar03@unimerz.ac.id)

Miladiarsi, Universitas Megarezky

Miladiarsi is a lecturer at Biomedical Science Department. She is Head of Biomedical Science Study Program at Megarezky University. Her research interest is in medical microbiology, and genetic epidemiology (email: miladiarsi.bio09@gmail.com).

Ade Irma, Universitas Megaresky

Ade Irma is a lecturer at Biomedical Science Department, Megarezky University. Her position is as Quality Control Taskforce in this university. She is active to promote education quality in health and medical sciences. (email: adeirmabio93@gmail.com).

Tenri Ayu Adri, Universitas Megarezky

Tenri Ayu Adri is a lecturer at Pharmacy Department, Megarezky University. Her position is as Head of Quality Assurance Unit at this department. She is active in many profesional association. (email: farmakologitenriayu@unimerz.ac.id).

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